Neuroinflammatory astrocyte subtypes in the mouse brain

Astrocytes undergo an inflammatory transition after infections, acute injuries and chronic neurodegenerative diseases. How this transition is affected by time and sex, its heterogeneity at the single-cell level and how sub-states are spatially distributed in the brain remains unclear. In this webcast, Dr. Shane Liddelow of NYU Langone’s Neuroscience Institute will present a transcriptomic investigation of mouse cortical astrocytes after an acute inflammatory stimulus.

The study identifies fast transcriptomic changes in astrocytes occurring within hours that drastically change over time. Sequencing ~80,000 astrocytes at single-cell resolution shows that inflammation causes a widespread response with subtypes of astrocytes undergoing distinct inflammatory transitions with defined transcriptomic profiles. The work also attributes key sub-states of inflammation-induced reactive astrocytes to specific brain regions using spatial transcriptomics and in situ hybridization.

Together, these datasets provide a powerful resource for profiling astrocyte heterogeneity and will be useful for understanding the biological importance of regionally constrained reactive astrocyte sub-states.

You will:

• Understand the diversity and heterogeneity of CNS astrocytes
• Discover how single-cell transcriptomics can be leveraged to assess the heterogenous response of cell subsets undergoing distinct reactions to pathological stimuli
• Learn how different astrocyte subtypes are distributed in the brain

Register today:  https://bit.ly/3PrPUWB

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